| Neu Isenburg to Nymburk | 281.03 mi (452.27 km) |
| Neu Isenburg to Olomouc | 382.25 mi (615.18 km) |
| Neu Isenburg to Opava | 408.65 mi (657.67 km) |
| Neu Isenburg to Orlova | 432.54 mi (696.10 km) |
| Neu Isenburg to Ostrava | 426.50 mi (686.38 km) |
| Neu Isenburg to Ostrov | 188.38 mi (303.17 km) |
| Neu Isenburg to Otrokovice | 399.20 mi (642.46 km) |
| Neu Isenburg to Pardubice | 314.00 mi (505.33 km) |
| Neu Isenburg to Pelhrimov | 294.30 mi (473.63 km) |
| Neu Isenburg to Pisek | 248.81 mi (400.42 km) |
| Neu Isenburg to Plzen | 209.19 mi (336.65 km) |
| Neu Isenburg to Podebrady | 284.49 mi (457.84 km) |
| Neu Isenburg to Policka | 337.47 mi (543.11 km) |
| Neu Isenburg to Prachatice | 248.07 mi (399.22 km) |
| Neu Isenburg to Prague | 253.69 mi (408.27 km) |
