| Gainesville to Olympia Heights (Florida) | 299.65 mi (482.24 km) |
| Gainesville to Omaha (Nebraska) | 1,108.70 mi (1,784.28 km) |
| Gainesville to On Top of the World Designated Place (Florida) | 39.86 mi (64.15 km) |
| Gainesville to Onalaska (Wisconsin) | 1,095.74 mi (1,763.43 km) |
| Gainesville to Oneida (New York) | 996.63 mi (1,603.92 km) |
| Gainesville to Oneonta (New York) | 970.82 mi (1,562.38 km) |
| Gainesville to Ontario (California) | 2,080.70 mi (3,348.57 km) |
| Gainesville to Ontario (Oregon) | 2,134.34 mi (3,434.89 km) |
| Gainesville to Opa-locka (Florida) | 290.79 mi (467.98 km) |
| Gainesville to Opelika (Alabama) | 272.96 mi (439.29 km) |
| Gainesville to Opelousas (Louisiana) | 584.68 mi (940.95 km) |
| Gainesville to Orange (California) | 2,093.55 mi (3,369.24 km) |
| Gainesville to Orange (Texas) | 684.10 mi (1,100.95 km) |
| Gainesville to Orange City (Florida) | 81.95 mi (131.88 km) |
| Gainesville to Orangeburg (South Carolina) | 277.41 mi (446.44 km) |
